lox antibody  (Bio-Techne corporation)

Journal: bioRxiv

Article Title: Expression of Lysyl Oxidase Family Enzymes During Human Endometrial Decidualization

doi: 10.1101/2025.05.28.656636

Figure Lengend Snippet: Immunohistochemical staining of LOX and LOXL1-4 in human endometrial tissues collected during the proliferative and secretory phases of the menstrual cycle. Representative images from six independent samples tested. Myometrial tissues served as a positive control. Imaging settings were customized for each individual image to optimize visualization. Scale bar: 100μm.

Article Snippet: The primary antibodies used were LOX antibody (Novus, NB100-2527, 1:100), LOXL1 antibody (ThermoFisher, Scientific PA5-87701, 1:100), LOXL2 antibody (Abcam, ab96233, 1:100), LOXL3 antibody (ThermoFisher, Scientific PA5-48462, 1:100), and LOXL4 antibody (ThermoFisher Scientific, PA5-58436, 1:100).

Techniques: Immunohistochemical staining, Staining, Positive Control, Imaging

Journal: bioRxiv

Article Title: Expression of Lysyl Oxidase Family Enzymes During Human Endometrial Decidualization

doi: 10.1101/2025.05.28.656636

Figure Lengend Snippet: A. Gene expression of LOX and LOXL1-4 in the human endometrial stromal cells decidualized in vitro . Primary human endometrial stromal cells were cultured in vitro until they reached full confluence. After that, the decidualization was induced with medium containing a hormonal cocktail: 10nM E, 1μM P, and 0.5mM 8-bromo-cAMP for 6 days. The cell lysates were collected at 0, 2, 4 and 6 days to extract total RNA for gene expression analysis. The gene expression was normalized to RPLPO and compared with day 0 samples (n=4/group, *p<0.05). B . Western blot analysis of LOX and LOXL1-3 protein levels in cell lysates collected at 0, 2, 4 and 6 days. GAPDH was used as a loading control. These are representative images from three to four independent replicates. C . Quantification of protein levels of LOX and LOXL1-3 is shown in histogram (n=3/group, *p<0.05).

Article Snippet: The primary antibodies used were LOX antibody (Novus, NB100-2527, 1:100), LOXL1 antibody (ThermoFisher, Scientific PA5-87701, 1:100), LOXL2 antibody (Abcam, ab96233, 1:100), LOXL3 antibody (ThermoFisher, Scientific PA5-48462, 1:100), and LOXL4 antibody (ThermoFisher Scientific, PA5-58436, 1:100).

Techniques: Gene Expression, In Vitro, Cell Culture, Western Blot, Control

Journal: bioRxiv

Article Title: Expression of Lysyl Oxidase Family Enzymes During Human Endometrial Decidualization

doi: 10.1101/2025.05.28.656636

Figure Lengend Snippet: A. Western blot analysis of LOX and LOXL1-3 protein levels in the conditioned media collected at 0, 2, 4 and 6 days after the induction of decidualization. These are representative images from three independent replicates. Note: The culture medium was replaced every 48 hours. PonceauS stain was used to assess transfer efficiency and to visualize total proteins. B. Quantification of protein levels of LOX and LOXL1-3 is shown in histogram (n=3/group, *p<0.05).

Article Snippet: The primary antibodies used were LOX antibody (Novus, NB100-2527, 1:100), LOXL1 antibody (ThermoFisher, Scientific PA5-87701, 1:100), LOXL2 antibody (Abcam, ab96233, 1:100), LOXL3 antibody (ThermoFisher, Scientific PA5-48462, 1:100), and LOXL4 antibody (ThermoFisher Scientific, PA5-58436, 1:100).

Techniques: Western Blot, Staining